cd105  (R&D Systems)

Journal: The FASEB Journal

Article Title: Bone Marrow Mesenchymal Stem Cell‐Derived Exosomes Promote M2 Polarization and Protect Against Acute Lung Injury

doi: 10.1096/fj.202502772R

Figure Lengend Snippet: Preparation of mouse BMSCs and their exosomes. (A) Morphological observation of mouse BMSCs at 120× magnification. To ensure objectivity, five random fields were selected for observation in each analysis. (B) Flow cytometry analysis of the expression of surface markers CD105, CD90, CD73 and CD34 in passage 2 BMSCs. (C) SEM characterization of BMSCs‐Exos. (D) Particle size analysis of BMSCs‐Exos using NTA. (E) Western blot analysis of CD9, CD63, and CD81 protein expression in BMSCs‐Exos, with lysis buffer used as a negative control. Three biological replicates were performed for the experiment ( n = 3), and each replicate was tested three times.

Article Snippet: For flow cytometric analysis, cells were fixed with 1% methanol, blocked with 1% BSA and 0.1% goat serum, incubated with antibodies anti‐mouse CD105, CD90, CD73 and CD34 (ab314950/ab25322/FAB4488V/ab23830; Abcam, Cambridge, UK or R&D Systems, MN, USA) each diluted 1:250, then washed and resuspended in Dulbecco's Phosphate‐Buffered Saline (dPBS).

Techniques: Flow Cytometry, Expressing, Particle Size Analysis, Western Blot, Lysis, Negative Control

Journal: Cell Transplantation

Article Title: Adipose-derived stem cells inhibit dendritic cell migration by secreting tumor necrosis factor-α-stimulated gene 6 to improve the allogeneic skin transplantation survival rate in mice

doi: 10.1177/09636897251376125

Figure Lengend Snippet: Characteristics of adipose-derived stem cells. (a) Positive expression for CD90 and CD105 and negative expression for CD19 and CD34. (b), (c) Differentiation of ADSCs into adipocytes and osteoblasts. After adipogenic differentiation, newly differentiated adipocytes had lipid droplets identified by Oil Red O staining. Osteogenic differentiation was confirmed by Alizarin Red S staining. ADSCs: adipose-derived stem cells; CD: cluster of differentiation.

Article Snippet: Passage 3 cells were stained with antibodies against CD90 (E-AB-F1283C) (Elabscience, Wuhan, China), CD105 (E-AB-F1233UE) (Elabscience, Wuhan, China), CD19 (E-AB-F1004E) (Elabscience, Wuhan, China), and CD34 (E-AB-F1284D) (Elabscience, Wuhan, China).

Techniques: Derivative Assay, Expressing, Staining